Parallel Secretion of Pancreatic Phospholipase A2, Phospholipase A1, Lipase, and Colipase in Children with Exocrine Pancreatic Dysfunction
نویسندگان
چکیده
منابع مشابه
Hydrolysis of milk fat globules by pancreatic lipase. Role of colipase, phospholipase A2, and bile salts.
Human milk fat globules require colipase to be hydrolyzed by pancreatic lipase in the presence of bile salts. This is contrary to a recent report in this Journal (J. Clin. Invest. 67: 1748-1752.) according to which inhibition of lipase by bile salt could be overcome by the addition of colipase or phospholipase A2. This latter finding is shown to be due to contamination of commercially available...
متن کاملHydrolysis of human milk fat globules by pancreatic lipase: role of colipase, phospholipase A2, and bile salts.
Human milk fat globules were used to explore how dietary triglycerides are hydrolyzed by pancreatic lipase. These triglycerides were hydrolyzed very slowly by lipase alone as if the surface layer of proteins and phospholipids impeded the action of the enzyme. The inhibition of lipase activity could be overcome by addition either of colipase or of pancreatic phospholipase A2. Colipase enhanced t...
متن کاملStructure and properties of a human non-pancreatic phospholipase A2.
We have purified a human non-pancreatic phospholipase A2 that is present in platelets and is enriched in rheumatoid synovial fluid. The enzyme is calcium-dependent, has a pH optimum of 8-10, and shows a striking preference for substrate presented in the form of Escherichia coli membranes. In the E. coli phospholipase A2 assay the phospholipase exhibits an apparent specific activity of 300 mumol...
متن کاملPurification and characterization of human pancreatic phospholipase A2.
We purified human pancreatic phospholipase A2 from postmortem pancreatic tissue by elution of the semi-purified enzyme on CM-Sephadex C-25 with a linear NaCl gradient at pH 6.0. The enzyme appeared as a single polypeptide chain with an isoelectric point of 9.2 +/- 0.1. The relative molecular mass of the enzyme was estimated to be 15 800 +/- 1000 by sodium dodecyl sulfate/polyacrylamide gel elec...
متن کاملSequence of a cDNA coding for bovine pancreatic phospholipase A2.
Phospholipaae A2 (PLA2) from pancreas has the following interesting features. The enzyme preferably acts on aggregated phospholipid micell-like structures, requires Ca + + for its activity, and is produced as a zymogen (1). To elucidate the relationship between these features and the structure of this enzyme, the gene encoding it and the expression system will be a useful tool. We have isolated...
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ژورنال
عنوان ژورنال: Pediatric Research
سال: 2000
ISSN: 0031-3998,1530-0447
DOI: 10.1203/00006450-200012000-00006